| ◈◈◈ Abstract Example ◈◈◈ |
 Title |
The Effect of Cisplatin on the Calcium Regulation in Isolated Outer Hair Cells |
|
| Purpose |
In this study, authors aimed to know the changes of calcium channel regulation by cisplatin in isolated outer hair cells and to speculate the mechanism of hearing loss by cisplatin. |
|
| Materials and Methods |
Outer hair cells were isolated from guinea pig after enzymatic and mechanic dissociation. Calcium current was recorded by whole cell patch clamp method and intracellular calcium was measured by Fura 2-AM fluorescent dye. Cisplatin solution (0.3 mM) was perfused in bath solution and the changes of calcium current and intracellular calcium were recorded. |
|
| Results |
In outer hair cell, calcium current was decreased after cisplatin perfusion, but the shape of current-voltage curve did not change. Intracellular calcium was also decreased after cisplatin perfusion, but after repeated perfusion, intracellular calcium increased even after perfusion with nifedipine. |
|
| Conclusion |
Calcium channel can be blocked and intracellular calcium store may be disturbed by cisplatin. Therefore, repeated exposure of cisplatin can increase intracellular calcium and make the cells dead. |
|